Table 1 Comparison of different methods for the preparation of testicular cell suspensions
Lam et al. 1970 | Meistrich 1972 | Malkov et al. 1998 | This work | |
|---|---|---|---|---|
Duration | FAST (~30 min) | Time-consuming (~1 h) | Time-consuming (~2 h) | Very fast (~15 min) |
Handling | Significant | High | High | Minimal |
Reproducibility | Variable | Variable | Variable | Very high |
Cell debris | Significant | Moderate | Not shown | Scarce |
Multinucleates | Significant | Significant | Significant | Scarce |
Viability | ≤80% | 98% | Not determined | >85% |
RNAses | No | No | Yes | No |
Trypsin/collagenase | No | Yes | Yes | No |