Table 1 Forward and reverse degenerate oligonucleotides to clone tiGHR I probe
Oligonucleotides | Sequences |
|---|---|
A | 5'...(tc)t(ag)aa(ct)tggac(acgt)(tc)t(ag)tt(ag)aa(ct)at...3' |
B | 5'...ct(tc)aa(ct)tggac(acgt)tt(ag)(ct)t(ag)aa(ct)at...3' |
C | 5'...(ct)t(ag)aa(ct)tggac(acgt)ct(tc)ct(tcag)aa(tc)at...3' |
D | 5'...ct(tc)aa(ct)tggac(acgt)ct(tcag)ct(tc)aa(tc)at...3' |
E | 5'...gg(ga)tc(tag)at(tg)cc(tc)tt(tga)at(tc)tt(tg)gg...3' |
F | 5'...gg(ga)tc(tga)at(tg)cc(tc)tt(tga)at(tc)tt(ca)gg...3' |
G | 5'...gg(ga)tc(tag)at(ca)cc(tc)tt(tga)at(tc)tt(tg)gg...3' |
H | 5'...gg(ag)tc(tag)at(ca)cc(tc)tt(tga)at(tc)tt(ca)gg...3' |