Figure 5
Determination of the quantity of tiGHR I RNA in tilapia liver. Lineal regressions with data from three different tilapias. The picture is an example of 2% agarose gel with the amplification products of competitive RT-PCR using total RNA from the liver of tilapia 4 and known quantities of competitor RNA. 1, molecular weight marker (leader 100 bp, Promega); 2, RT-PCR negative control; 3, RT-PCR with 5 × 105 molecules of RNA competitor alone; 4, RT-PCR with liver total RNA of tilapia alone; 5, 6, and 7, RT-PCR of liver total RNA of tilapia 4 with 3 × 105, 6 × 105, and 8 × 105 molecules of competitor, respectively.