Figure 4
Lineal regressions generated from the densitometry data of the PCR reaction using fixed target molecule numbers and serial dilutions of competitor. In the picture, typical 2% gel image of amplification products in the competitive PCRs where 105 molecules of target were used with serial dilutions of competitor. 1, PCR negative control; lanes 2–4, 104; lanes 5–7, 5 × 104; lanes 8–10, 7.5 × 104; lanes 11–13, 105; lanes 14–16, 5 × 105; lanes 17–19, 7.5 × 105 molecules of competitor, respectively; 20, molecular weight marker (from top to bottom = 1.4 kb, 1.2 kb, 750 bp, 450 bp, and 366 bp).