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Featured Article: Breaking the Mucin Barrier: A New Affinity Chromatography-Mass Spectrometry Approach to Unveil Potential Cell Markers and Pathways Altered in Pseudomyxoma Peritonei

New Content ItemThis work describes the first protocol capable of breaking the mucin barrier to access and isolate the protein fraction from mucinous tumours through a combination of homogenization, liquid chromatography to reduce mainly glycoproteins, albumins and immunoglobulin G, and mass spectrometry analysis. Thus, we present here the first proteome analysed in Pseudomyxoma peritonei (PMP),  the identification of a distinct mucin isoform profile in soft compared to hard mucinous tumour tissue, the enrichment of membrane-associated mucins as well as key biological processes/pathways altered in mucinous tumours. Importantly, this protocol also allowed us to identify MUC13 as a potential tumour cell marker in PMP.

In conclusion, we believe that our study provides essential, original information to facilitate rapid advances in the knowledge of PMP pathogenesis, which is undoubtedly the first step towards the development of new and effective therapeutic tools for the treatment mucinous neoplasms.

Image courtesy of the authors.

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Biological Procedures Online publishes articles that enhance access to techniques and improve basic understanding of medical and biological sciences. We are particularly interested in important research discoveries in technology development, animal models of human diseases, and cancer biology and therapeutic approaches.

Biological Procedures Online places emphasis on multidisciplinary approaches that integrate novel methodologies and mechanism understanding from medicine, biology, imaging, engineering, bioinformatics, and system analysis.

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About the Editor

Dr. Bolin Liu, Editor-in-Chief

Dr. Bolin Liu is Professor in the Departments of Interdisciplinary Oncology and Genetics and the Stanley S. Scott Cancer Center at School of Medicine, Louisiana State University (LSU) Health Sciences Center-New Orleans. He earned his MD from Beijing Medical University and received extensive postdoctoral training in cancer biology at MD Anderson Cancer Center. Dr. Liu became an independent investigator in the Department of Pathology at University of Colorado Anschutz Medical Campus in 2007. He was recruited to his current institution in 2018. Dr. Liuā€™s research interests focus on receptor tyrosine kinases (RTKs)-initiated cell signaling in drug resistance and tumor metastasis, with a hope to identify novel molecular targets and develop epigenetic approaches for cancer treatment. Dr. Liu has published over 80 peer-reviewed articles in the field of cancer research, and received several honors and awards, including a Research Award from Susan G. Komen for the Cure (2007), an Elsa U. Pardee Foundation Award (2014), and a Translational Research Award from METAvivor Research and Support, Inc. (2020).

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