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  1. The use of architectural and morphological characteristics of cells for establishing prognostic indicators by which individual pathologies are assigned grade and stage is a well-accepted practice. Advances in ...

    Authors: John N McGinley, Matthew D Thompson and Henry J Thompson
    Citation: Biological Procedures Online 2010 12:118
  2. There are several traditional and well-developed methods for analyzing the activity of transcription factors, such as EMSA, enzyme-linked immunosorbent assay, and reporter gene activity assays. All of these me...

    Authors: Yu-Ling Lin, Yun-Ju Lai, Nu-Man Tsai, Tai-Chu Peng, Yen-Ku Liu, Ru-Ping Lee, Chueh-Jen Tsai and Kuang-Wen Liao
    Citation: Biological Procedures Online 2010 12:18
  3. Development of antimitotic binding to the colchicine-binding site for the treatment of cancer is rapidly expanding. Numerous antimicrotubule agents are prepared every year, and the determination of their bindi...

    Authors: Sébastien Fortin, Jacques Lacroix, Marie-France Côté, Emmanuel Moreau, Éric Petitclerc and René C-Gaudreault
    Citation: Biological Procedures Online 2010 12:113
  4. Indoleamine 2,3 dioxygenase (IDO) is a potent immunomodulatory enzyme that has recently attracted significant attention for its potential application as an inducer of immunotolerance in transplantation. We hav...

    Authors: Alireza Moeen Rezakhanlou, Darya Habibi, Amy Lai, Reza B Jalili, Christopher J Ong and Aziz Ghahary
    Citation: Biological Procedures Online 2010 12:107
  5. Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity a...

    Authors: Marcello Tagliavia, Anna Taravella, Sandra Marineo, Anna Maria Puglia and Mario La Farina
    Citation: Biological Procedures Online 2010 12:27
  6. Laser capture microdissection (LCM) enables collection of cell populations highly enriched for specific cell types that have the potential of yielding critical information about physiological and pathophysiolo...

    Authors: John N McGinley, Zongjian Zhu, Weiqin Jiang and Henry J Thompson
    Citation: Biological Procedures Online 2010 12:31
  7. The aim of this study was to compare different cell sources and culture conditions to obtain endothelial progenitor cells (EPCs) with predictable antigen pattern, proliferation potential and in vitro vasculoge...

    Authors: Claudio Muscari, Chiara Gamberini, Ilaria Basile, Francesca Bonafé, Simond Valgimigli, Ombretta Capitani, Carlo Guarnieri and Claudio Marcello Caldarera
    Citation: Biological Procedures Online 2010 12:89
  8. The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide...

    Authors: Nakul Mandal, Steffen Heegaard, Jan Ulrik Prause, Bent Honoré and Henrik Vorum
    Citation: Biological Procedures Online 2009 12:56
  9. Subtraction technique has been broadly applied for target gene discovery. However, most current protocols apply relative differential subtraction and result in great amount clone mixtures of unique and differe...

    Authors: Ning Wu, Kanyand Matand and Sonya Williams
    Citation: Biological Procedures Online 2009 12:44
  10. Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hy...

    Authors: Anita Gothelf, Pernille Hojman and Julie Gehl
    Citation: Biological Procedures Online 2009 11:325
  11. Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor of PKCδ, can inhibit many other PKC-unrelated kinases and has a numb...

    Authors: Emanuela Maioli, Claudia Torricelli, Vittoria Fortino, Filippo Carlucci, Valentina Tommassini and Adriana Pacini
    Citation: Biological Procedures Online 2009 11:227
  12. Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas...

    Authors: Jeffrey D Carter, Stacey B Dula, Kathryn L Corbin, Runpei Wu and Craig S Nunemaker
    Citation: Biological Procedures Online 2009 11:3
  13. The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of serious pathological states such as cancer and fibrosis. In t...

    Authors: Andrea Staršíchová, Lukáš Kubala, Eva Lincová, Zuzana Pernicová, Alois Kozubík and Karel Souček
    Citation: Biological Procedures Online 2009 11:316
  14. MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of targ...

    Authors: Fredy Siegrist, Thomas Singer and Ulrich Certa
    Citation: Biological Procedures Online 2009 11:113
  15. In the image segmentation process of positron emission tomography combined with computed tomography (PET/CT) imaging, previous works used information in CT only for segmenting the image without utilizing the i...

    Authors: Iman Avazpour, M Iqbal Saripan, Abdul Jalil Nordin and Rajaa Syamsul Azmir Iman Abdullah
    Citation: Biological Procedures Online 2009 11:241
  16. Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effecti...

    Authors: Adria D Dismuke, Aimee D Kohn, Randall T Moon and Melissa H Wong
    Citation: Biological Procedures Online 2009 11:130
  17. Many species of streptococci secrete and use a competence-stimulating peptide (CSP) to initiate quorum sensing for induction of genetic competence, bacteriocin production, and other activities. These signaling...

    Authors: XiaoLin Tian, Raymond T Syvitski, TianLei Liu, Nadine Livingstone, David L Jakeman and Yung-Hua Li
    Citation: Biological Procedures Online 2009 11:207
  18. Evaluation of blood smear is a commonly clinical test these days. Most of the time, the hematologists are interested on white blood cells (WBCs) only. Digital image processing techniques can help them in their...

    Authors: Farnoosh Sadeghian, Zainina Seman, Abdul Rahman Ramli, Badrul Hisham Abdul Kahar and M-Iqbal Saripan
    Citation: Biological Procedures Online 2009 11:196
  19. Comparative genomics has become a real tantalizing challenge in the postgenomic era. This fact has been mostly magnified by the plethora of new genomes becoming available in a daily bases. The overwhelming lis...

    Authors: Jennifer Commins, Christina Toft and Mario A Fares
    Citation: Biological Procedures Online 2009 11:52
  20. The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-fre...

    Authors: Boon C Heng, Catherine M Cowan and Shubhayu Basu
    Citation: Biological Procedures Online 2009 11:161
  21. Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for...

    Authors: Alina Rodríguez-Mallon, Yamilet Cárdenas, Juana María Lugo, Aymé Oliva, Antonio Morales and Mario Pablo Estrada
    Citation: Biological Procedures Online 2009 11:79
  22. As many as 50% of adults with type I (T1) diabetes exhibit bone loss and are at increased risk for fractures. Therapeutic development to prevent bone loss and/or restore lost bone in T1 diabetic patients requi...

    Authors: Katherine Motyl and Laura R McCabe
    Citation: Biological Procedures Online 2009 11:296
  23. Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the complex organization of the mammalian testicular tissue, var...

    Authors: Rosana Rodríguez-Casuriaga, Adriana Geisinger, Beatriz López-Carro, Valentina Porro, Rodolfo Wettstein and Gustavo A Folle
    Citation: Biological Procedures Online 2009 11:184
  24. The present study describes a protocol to generate heterogenous populations of neurotransmitter-producing neurons from human mesenchymal stem cells (MSCs). MSCs are bone marrow (BM)-derived cells which undergo...

    Authors: Steven J. Greco, Chunyi Zhou, Jiang-Hong Ye and Pranela Rameshwar
    Citation: Biological Procedures Online 2008 10:101000090
  25. The early chick embryo contains subpopulations of cells that express lineage-specific transcription factors. We have developed protocols to examine the role of these cells during development that involve label...

    Authors: Jacquelyn Gerhart, Christine Neely, Jessica Pfautz and Mindy George-Weinstein
    Citation: Biological Procedures Online 2008 10:101000074
  26. Gene families are widely used in comparative genomics, molecular evolution, and in systematics. However, they are constructed in different manners, their data analyzed and interpreted differently, with differe...

    Authors: David A. Liberles and Katharina Dittmar
    Citation: Biological Procedures Online 2008 10:101066
  27. DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study s...

    Authors: Alexander Unterberger, Jérôme Torrisani and Moshe Szyf
    Citation: Biological Procedures Online 2008 10:101047
  28. Acetyl phosphate (acetyl-P) serves critical roles in coenzyme A recycling and ATP synthesis. It is the intermediate of the Pta-AckA pathway that inter-converts acetyl-coenzyme A and acetate. Acetyl-P also can ...

    Authors: David H. Keating, Ana Shulla, Adam H. Klein and Alan J. Wolfe
    Citation: Biological Procedures Online 2008 10:101036
  29. Calcitriol (1α, 25-dihydroxyvitamin D3) induces the expression of CD14 in mononuclear phagocytes. The mechanisms accounting for this have been unclear since the promoter of CD14 does not contain a canonical vitam...

    Authors: Alireza Moeenrezakhanlou, Devki Nandan and Neil E. Reiner
    Citation: Biological Procedures Online 2008 10:101029
  30. Understanding mechanisms of gene regulation has broad therapeutic implications for human disease. Here we describe a novel method for generating human cell lines that serve as reporters of transcriptional acti...

    Authors: Sandra L. Fernandez, David W. Russell and Peter J. Hurlin
    Citation: Biological Procedures Online 2007 9:91084
  31. Recent advances in redox proteomics have provided significant insight into the role of oxidative modifications in cellular signalling and metabolism. At present, these techniques rely heavily on Western blots ...

    Authors: H. Fai Poon, Laila Abdullah, Jon Reed, Sarah M. Doore, Cyndi Laird, Venkat Mathura, Michael Mullan and Fiona Crawford
    Citation: Biological Procedures Online 2007 9:91065
  32. In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. M...

    Authors: Nitixa Patel, Marianne Castillo and Pranela Rameshwar
    Citation: Biological Procedures Online 2007 9:91056
  33. Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess differ...

    Authors: Irene Salinas, José Meseguer and Maria Ángeles Esteban
    Citation: Biological Procedures Online 2007 9:91043
  34. We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cass...

    Authors: Seok Hwee Koo, Tan Ching Ong, Kok Ting Chong, Caroline Guat Lay Lee, Fook Tim Chew and Edmund Jon Deoon Lee
    Citation: Biological Procedures Online 2007 9:91027
  35. We developed a rapid mutagenesis method based on a modification of the QuikChange® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag. The modificatio...

    Authors: Qiwen Deng, Wensheng Luo and Michael S. Donnenberg
    Citation: Biological Procedures Online 2007 9:91018
  36. Suppression of gene expression by small interfering RNA (siRNA) has proved to be a gene-specific and cost effective alternative to other gene suppression technologies. Short hairpin RNAs (shRNAs) generated fro...

    Authors: Hailong Wu, Anh Dinh and Yin-Yuan Mo
    Citation: Biological Procedures Online 2007 9:91009
  37. We have developed a system to visualize functionally activated neurons and their projections in the brain. This system utilizes a transgenic mouse, fos-tau-lacZ (FTL), which expresses the marker gene, lacZ, in ne...

    Authors: Mark Murphy, Ursula Greferath and Yvette M. Wilson
    Citation: Biological Procedures Online 2007 9:91001

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