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  1. Short report

    A BioBrick™-Compatible Vector for Allelic Replacement Using the XylE Gene as Selection Marker

    Circular plasmid-mediated homologous recombination is commonly used for marker-less allelic replacement, exploiting the endogenous recombination machinery of the host. Common limitations of existing methods in...

    Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolò Politi, Ilaria Massaiu, Cinzia Calvio, Maria Gabriella Cusella De Angelis and Paolo Magni

    Biological Procedures Online 2016 18:6

    Published on: 13 February 2016

  2. Review

    Lipase catalysis in organic solvents: advantages and applications

    Lipases are industrial biocatalysts, which are involved in several novel reactions, occurring in aqueous medium as well as non-aqueous medium. Furthermore, they are well-known for their remarkable ability to c...

    Ashok Kumar, Kartik Dhar, Shamsher Singh Kanwar and Pankaj Kumar Arora

    Biological Procedures Online 2016 18:2

    Published on: 13 January 2016

  3. Methodology

    A Medium-Throughput Single Cell CRISPR-Cas9 Assay to Assess Gene Essentiality

    Target selection for oncology is a crucial step in the successful development of therapeutics. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 editing of specific loci offers an alterna...

    A. R. Grassian, T. M. E. Scales, S. K. Knutson, K. W. Kuntz, N. J. McCarthy, C. E. Lowe, J. D. Moore, R. A. Copeland, H. Keilhack, J. J. Smith, J. A. Wickenden and S. Ribich

    Biological Procedures Online 2015 17:15

    Published on: 14 November 2015

  4. Research

    Mitogen Activated Protein Kinase (MPK) Interacts With Auxin Influx Carrier (OsAux/LAX1) Involved in Auxin Signaling in Plant

    Mitogen activated protein kinases (MPKs) are serine/threonine protein kinases that contain characteristic T-x-Y motif in the activation loop region. MPKs are important signaling molecules involved in diverse s...

    Tapan Kumar Mohanta, Nibedita Mohanta, Pratap Parida and Hanhong Bae

    Biological Procedures Online 2015 17:13

    Published on: 30 October 2015

  5. Review

    The diversity of fungal genome

    The genome size of an organism varies from species to species. The C-value paradox enigma is a very complex puzzle with regards to vast diversity in genome sizes in eukaryotes. Here we reported the detailed ge...

    Tapan Kumar Mohanta and Hanhong Bae

    Biological Procedures Online 2015 17:8

    Published on: 2 April 2015

  6. Methodology

    cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit

    The synthesis of complementary DNA (cDNA) for use in the detection of BCR-ABL1 at the Major Molecular Response (MMR) level is a well-established method used by clinical laboratories world-wide. However, the quali...

    Jianxiang Chi, Chryso Pierides, Andrie Mitsidou, Andri Miltiadou, Petroula Gerasimou and Paul Costeas

    Biological Procedures Online 2015 17:4

    Published on: 9 February 2015

  7. Research

    Evaluation of method performance for oxidative stress biomarkers in urine and biological variations in urine of patients with type 2 diabetes mellitus and diabetic nephropathy

    Oxidative stress biomarkers such as superoxide dismutase (CuZnSOD), catalase (CAT) and malondialdehyde (MDA) play an important role in the pathogenesis or progression of numerous diseases. Data regarding the b...

    Ergul Belge Kurutas, Yakup Gumusalan, Ali Cetinkaya and Ekrem Dogan

    Biological Procedures Online 2015 17:3

    Published on: 2 February 2015

  8. Methodology

    Comparison of Vibratome and Compresstome sectioning of fresh primate lymphoid and genital tissues for in situ MHC-tetramer and immunofluorescence staining

    For decades, the Vibratome served as a standard laboratory resource for sectioning fresh and fixed tissues. In skilled hands, high quality and consistent fresh unfixed tissue sections can be produced using a V...

    Hadia M Abdelaal, Hyeon O Kim, Reece Wagstaff, Ryoko Sawahata, Peter J Southern and Pamela J Skinner

    Biological Procedures Online 2015 17:2

    Published on: 7 January 2015

  9. Review

    Model of Cation Transportation Mediated by High-Affinity Potassium Transporters (HKTs) in Higher Plants

    Trk/Ktr/HKT transporters probably were evolved from simple K+ channels KcsA. HKT transporters, which mediate Na+-uniport or Na+/K+-symport, maintain K+/Na+ homeostasis and increase salinity tolerance, can be clas...

    Yi Su, Weigui Luo, Wanhuang Lin, Liying Ma and Mohammed Hunayun Kabir

    Biological Procedures Online 2015 17:1

    Published on: 6 January 2015

    The Erratum to this article has been published in Biological Procedures Online 2015 17:9

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