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Synthesis, purification and crystallization of guanine-rich RNA oligonucleotides

Biological Procedures Online volume 6, pages 257–262 (2004)Cite this article

Abstract

Guanine-rich RNA oligonucleotides display many novel structural motifs in recent crystal structures. Here we describe the procedures of the chemical synthesis and the purification of such RNA molecules that are suitable for X-ray crystallographic studies. Modifications of the previous purification methods allow us to obtain better yields in shorter time. We also provide 24 screening conditions that are very effective in crystallization of the guanine-rich RNA oligonucleotides. Optimal crystallization conditions are usually achieved by adjustment of the concentration of the metal ions and pH of the buffer. Crystals obtained by this method usually diffract to high resolution.

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Authors and Affiliations

  1. Departments of Chemistry and Biochemistry, The Ohio State University, 200 Johnston Laboratory, 43210, Columbus, OH

    Baocheng Pan, Ke Shi & Muttaiya Sundaralingam

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  1. Baocheng Pan
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  2. Ke Shi
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  3. Muttaiya Sundaralingam
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Correspondence to Muttaiya Sundaralingam.

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Published: November 22, 2004.

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Pan, B., Shi, K. & Sundaralingam, M. Synthesis, purification and crystallization of guanine-rich RNA oligonucleotides. Biol. Proced. Online 6, 257–262 (2004). https://doi.org/10.1251/bpo96

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  • DOI: https://doi.org/10.1251/bpo96

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Biological Procedures Online

ISSN: 1480-9222

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