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Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry

Biological Procedures Online volume 6, pages 220–225 (2004)Cite this article

Abstract

The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound’s toxicity is essential in the development of novel agents. Our interest is in a rapid, simple, high throughput screening method to assess cytotoxicity induced by potential agents. Some intracellular pathogens, such as Mycobacterium tuberculosis primary site of infection is human alveolar macrophages. Thus, evaluation of candidate drugs for macrophage toxicity is crucial. Protocols for high throughput drug toxicity screening of macrophages using flow cytometry are lacking in the literature. For this application we modified a preexisting technique, propidium iodide (PI) exclusion staining and utilized it for rapid toxicity tests. Samples were prepared in 96 well plates and analyzed by flow cytometry, which allowed for rapid, inexpensive and precise assessment of compound’s toxicity associated with cell death.

Abbreviations

FACS:

Fluorescence Activated Cell Sorting

PI:

propidium iodide

LD50 :

lethal dose in which 50% of the cells die

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Authors and Affiliations

  1. Department of Medicine, Division of Infectious Diseases, University of British Columbia, 2733 Heather Street, D450 HP East, V5Z 3J5, Vancouver, BC, Canada

    Neora Pick, Scott Cameron, Dorit Arad & Yossef Av-Gay

Authors
  1. Neora Pick
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  2. Scott Cameron
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  3. Dorit Arad
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  4. Yossef Av-Gay
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Corresponding author

Correspondence to Yossef Av-Gay.

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Published: October 1, 2004.

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Pick, N., Cameron, S., Arad, D. et al. Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry. Biol. Proced. Online 6, 220–225 (2004). https://doi.org/10.1251/bpo92

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  • DOI: https://doi.org/10.1251/bpo92

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Biological Procedures Online

ISSN: 1480-9222

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