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Dynamic force microscopy for imaging of viruses under physiological conditions

Biological Procedures Online volume 6, pages 120–128 (2004)Cite this article

Abstract

Dynamic force microscopy (DFM) allows imaging of the structure and the assessment of the function of biological specimens in their physiological environment. In DFM, the cantilever is oscillated at a given frequency and touches the sample only at the end of its downward movement. Accordingly, the problem of lateral forces displacing or even destroying bio-molecules is virtually inexistent as the contact time and friction forces are reduced. Here, we describe the use of DFM in studies of human rhinovirus serotype 2 (HRV2) weakly adhering to mica surfaces. The capsid of HRV2 was reproducibly imaged without any displacement of the virus. Release of the genomic RNA from the virions was initiated by exposure to low pH buffer and snapshots of the extrusion process were obtained. In the following, the technical details of previous DFM investigations of HRV2 are summarized.

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Authors and Affiliations

  1. Institute for Biophysics, J. Kepler University of Linz, Altenbergerstr. 69, A-4040, Linz, Austria

    Ferry Kienberger, Rong Zhu, Christian Rankl & Peter Hinterdorfer

  2. the Research Department Biomedical Nanotechnology, Upper Austrian Research GmbH, Scharitzerstr. 6-8, A-4020, Linz, Austria

    Rong Zhu

  3. Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Austria

    Rosita Moser & Dieter Blaas

  4. Department of Medical Biochemistry, University of Vienna, Dr. Bohr Gasse 9/3, A-1030, Vienna, Austria

    Rosita Moser & Dieter Blaas

Authors
  1. Ferry Kienberger
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  2. Rong Zhu
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  3. Rosita Moser
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  4. Christian Rankl
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  5. Dieter Blaas
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  6. Peter Hinterdorfer
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Correspondence to Peter Hinterdorfer.

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Published: June 29, 2004.

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Kienberger, F., Zhu, R., Moser, R. et al. Dynamic force microscopy for imaging of viruses under physiological conditions. Biol. Proced. Online 6, 120–128 (2004). https://doi.org/10.1251/bpo80

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  • DOI: https://doi.org/10.1251/bpo80

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Biological Procedures Online

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