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  • Open Access

Serum-free cryopreservation of five mammalian cell lines in either a pelleted or suspended state

Biological Procedures Online6:610061

  • Received: 5 November 2003
  • Accepted: 16 March 2004


Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using five mammalian lines (Crandell Feline Kidney, MCF7, A72, WI 38 and NB324K), we found that the serum-free alternative preserves nearly as efficiently as the serum-containing preservatives. Second, we compared cryostorage of those cells in suspended versus a pellet form using both aforementioned cryopreservatives. Under our conditions, cells were in general recovered equally well in a suspended versus a pellet form.

Indexing terms

  • Cryopreservation
  • Cells, Cultured
  • Culture Media, Serum-Free