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Serum-free cryopreservation of five mammalian cell lines in either a pelleted or suspended state

Abstract

Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using five mammalian lines (Crandell Feline Kidney, MCF7, A72, WI 38 and NB324K), we found that the serum-free alternative preserves nearly as efficiently as the serum-containing preservatives. Second, we compared cryostorage of those cells in suspended versus a pellet form using both aforementioned cryopreservatives. Under our conditions, cells were in general recovered equally well in a suspended versus a pellet form.

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Correspondence to Joe Corsini.

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Published: April 7, 2004.

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Corsini, J., Hacker, C. & Bare, C. Serum-free cryopreservation of five mammalian cell lines in either a pelleted or suspended state. Biol. Proced. Online 6, 61–66 (2004). https://doi.org/10.1251/bpo73

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  • DOI: https://doi.org/10.1251/bpo73

Indexing terms

  • Cryopreservation
  • Cells, Cultured
  • Culture Media, Serum-Free