Open Access

Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes

  • Bianca Obermaier1,
  • Marc Dauer2,
  • Jan Herten1,
  • Katharina Schad1,
  • Stefan Endres1 and
  • Andreas Eigler3Email author
Biological Procedures Online5:51197

https://doi.org/10.1251/bpo62

Received: 18 August 2003

Accepted: 22 September 2003

Abstract

We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytesin vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake. We then compared two different strategies for DC maturation: proinflammatory mediators were either added together with GM-CSF and IL-4 from the beginning of cell culture or added after 24 hours of differentiation with GM-CSF and IL-4. After 48 hours of total culture period, expression of activation markers was more pronounced in cells generated by the 2-step differentiation and activation method. Our new protocol for 2-day DC differentiation reduces labor, cost and time and also reliably renders high numbers of mature and viable DCs.

Indexing terms

Monocytes Dendritic cells Antigen presenting cells Vaccination Cell differentiation

Notes

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