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Aequorin-based measurements of intracellular Ca2+-signatures in plant cells

Biological Procedures Online volume 4, pages 105–118 (2002)Cite this article

Abstract

Due to the involvement of calcium as a main second messenger in the plant signaling pathway, increasing interest has been focused on the calcium signatures supposed to be involved in the patterning of the specific response associated to a given stimulus. In order to follow these signatures we described here the practical approach to use the non-invasive method based on the aequorin technology. Besides reviewing the advantages and disadvantages of this method we report on results showing the usefulness of aequorin to study the calcium response to biotic (elicitors) and abiotic stimuli (osmotic shocks) in various compartments of plant cells such as cytosol and nucleus.

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Authors and Affiliations

  1. Department Biologie I der Ludwig-Maximilians-Universität, Botanik, Menzinger Str. 67, D-80638, München, Germany

    Axel Mithöfer

  2. Signaux et Messages Cellulaires chez les Végétaux, UMR CNRS/UPS 5546, 24 Chemin de Borde Rouge, BP 17 Auzeville, F-31326, Castanet-Tolosan, France

    Christian Mazars

Authors
  1. Axel Mithöfer
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  2. Christian Mazars
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Correspondence to Axel Mithöfer.

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Published: December 9, 2002

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Mithöfer, A., Mazars, C. Aequorin-based measurements of intracellular Ca2+-signatures in plant cells. Biol Proced Online 4, 105–118 (2002). https://doi.org/10.1251/bpo40

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  • DOI: https://doi.org/10.1251/bpo40

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Biological Procedures Online

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