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  • Open Access

Measurement of cardiac mechanical function in isolated ventricular myocytes from rats and mice by computerized video-based imaging

Biological Procedures Online3:31043

  • Received: 19 May 2001
  • Accepted: 30 October 2001


Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With the recent advances in cellular physiology and transgenic techniques, direct measurement of isolated ventricular myocyte mechanics is becoming an increasingly important technique in cardiac physiology that provides fundamental information on excitation-contraction coupling of the heart, either in drug intervention or pathological states. The goal of this article is to describe the isolation of ventricular myocytes from both rats and mice, and the use of real-time beat-to-beat simultaneous recording of both myocyte contraction and intracellular Ca2+ transients.

Indexing terms

  • myocyte
  • contractility
  • intracellular Ca2+ transients
  • edge detection