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Yeast two-hybrid: State of the art

Biological Procedures Online volume 2, pages 1–38 (1999)Cite this article

Abstract

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a “differential” screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to “real” antibodies. With the technology available, the only limitation is imagination.

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  1. DEVGEN N.V. Technologiepark 9, 9052, Zwijnaarde-Gent

    Wim Van Criekinge

  2. Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, B-9000, Ghent, Belgium

    Rudi Beyaert

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Van Criekinge, W., Beyaert, R. Yeast two-hybrid: State of the art. Biol Proced Online 2, 1–38 (1999). https://doi.org/10.1251/bpo16

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