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Yeast two-hybrid: State of the art

Abstract

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a “differential” screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to “real” antibodies. With the technology available, the only limitation is imagination.

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Correspondence to Wim Van Criekinge.

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Van Criekinge, W., Beyaert, R. Yeast two-hybrid: State of the art. Biol Proced Online 2, 1–38 (1999). https://doi.org/10.1251/bpo16

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Keywords

  • Yeast Cell
  • Reporter Gene
  • Biological Procedure
  • Library Plasmid
  • Upstream Activate Sequence