Fig. 4

BPTF deficiency affects apoptosis and the cell cycle of NB cell. A, C SK-N-BE (2) cells [mean, 16.2% vs. 20.2% vs. 25.2%, A] and SH-SY5Y cells [mean, 19.4% vs. 29.6% vs. 25.9, C] were analyzed by flow cytometry for Annexin V and 7AAD dual labeling representatively. Annexin V-positive cells were designated as apoptotic cells. B, D The proportions of apoptotic SK-N-BE (2) cells (B) and SH-SY5Y cells (D). E, G The percentage of SK-N-BE (2) cells (E) and SH-SY5Y cells (G) in different phases of the cell cycle was determined by FACS analysis with PI staining. F, H) Distribution of Cell Cycle Phases in SK-N-BE (2) cells [G0/G1: mean, 43.6% vs. 67.4% vs. 61.0, F] and SH-SY5Y cells [G0/G1: mean, 51.5% vs. 89.0% vs. 84.7, H]. Error bars represent SEM. The data are representative of at least three independent experiments. * P < 0.05, **** P < 0.0001, as assessed by Student’s t-tests