Fig. 3

BPTF leads to an increase in neuroblastoma cell migration and invasion. A-B SK-N-BE (2) cell migration following BPTF depletion (A) and using AU1 (B) shown by RTCA. Error bars represent SD. C-F Wound healing assay(0 h, 12 h, 24 h) to observe the migration of SK-N-BE (2) cell line and wound healing rates 24 h after scratch following BPTF knockdown [mean, 21.2% vs. 8.6%vs. 8.6%, C-D] and using AU1 [mean, 37.9% vs. 35.6% vs. 24.4% vs. 14.0%, E–F]. G-J Compare the invasion and migration of SK-N-BE (2) cell following BPTF downregulation [mean, 1.000 vs. 0.319 vs. 0.503, G; 1.000 vs. 0.387 vs. 0.648, H] and using AU1 by transwell [mean, 1.000 vs. 0.679 vs. 0.203 vs. 0.028, I; 1.000 vs. 0.441 vs. 0.105 vs. 0.005, J]. Error bars represent SEM. The data are representative of at least three independent experiments. ** P < 0.01, *** P < 0.001, **** P < 0.0001, as assessed by Student’s t-tests