Fig. 9

The regulatory role of NDRG1 in the growth and tumor metastasis of HCC. A-D A xenograft tumor model of LM3 cells showed that the volume and weight of the tumor were significantly reduced, and the growth rate of the tumor was significantly slowed after NDRG1 knockdown. E Immunohistochemical assays revealed that the expression levels of Ki67,Vimentin and GPX4 were inhibited and the expression levels of DHE was upregulated in HCC cells after NDRG1 knockdown. F-I The statistical results showed that knockdown of NDRG1 restricted the growth of HCC tumors. J-M We constructed a tail vein lung metastasis model, and the results of in vivo imaging (J), photography (K) and HE staining (L) demonstrated that the tumor size and number of HCC lung metastases were markedly decreased after NDRG1 knockdown