Fig. 8

NDRG1 inhibition induced HCC cells ferroptosis and contribute to RLS3-induced ferroptosis. A-B FCM and quantification analysis showing increased ROS expression after NDRG1 knockdown in SK-HEP1 and LM3 cells. C-F C11-BODIPY (a marker of lipid peroxidation) probe and quantification analysis showing increasing oxidized ROS expression in SK-HEP1 (CE) and LM3 (DF) cells after knockdown of NDRG1. G-H Expression levels of MDA after NDRG1 knockdown in SK-HEP1 (G) and LM3 (H) cells. I Representative images of mitochondria within SK-HEP1 and LM3 cells under TEM after NDRG1 knockdown. J-K Expression levels of iron ions after knockdown of NDRG1 in SK-HEP1 (G) and LM3 (H) cells. L-M Effect of different concentrations of RSL3 treatment on cell viability of SK-HEP1 and LM3 cells after knockdown of NDRG1