Fig. 7

Effects of NDRG1 knockdown on HCC cell proliferation and migration. A-B Western blot results showing the knockdown efficiency of NDRG1 shRNA in SK-HEP1 (A) and HCCLM3 (B) cells. C-D CCK8 experiments demonstrate the effect of NDRG1 knockdown on the proliferation of SK-HEP1 (C) and HCCLM3 (D) cells. E–F Live and dead cell staining demonstrated the effect of NDRG1 knockdown on SK-HEP1 (E) and HCCLM3 (F) cell survival. Ruler: 100 m. G EdU experiments showing the effect of NDRG1 knockdown on the proliferation of SK-HEP1 and HCCLM3 cells (**p < 0.01, ***p < 0.001). Ruler: 100 m. H Transwell cell migration assay showing the effect of NDRG1 knockdown on the migration of SK-HEP1 and HCCLM3 cells (***p < 0.001). Ruler: 100 m. I-J Immunofluorescence experiments showing altered PCNA expression (PCNA red) in SK-HEP1 (I) and HCCLM3 cells (J) after NDRG1 knockdown. K-L Immunofluorescence experiments showing altered expression of Vimentin (Vimentin red) in SK-HEP1 (K) and HCCLM3 cells (L) after NDRG1 knockdown. Ruler: 200 μm