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Fig. 4 | Biological Procedures Online

Fig. 4

From: Exosomal microRNA-342-5p secreted from adipose-derived mesenchymal stem cells mitigates acute kidney injury in sepsis mice by inhibiting TLR9

Fig. 4

AMSC-Exo-342 promotes cell autophagy to ameliorate LPS-induced cell inflammation. Notes: The HK-2 cells received autophagy activator Rapa or autophagy inhibitor 3-MA prior to LPS induction and exposure to AMSC-Exo-67 or AMSC-Exo-342. The fluorescence intensity of LC3 was measured by immunofluorescence staining (A), and the number of autophagosomes in HK-2 cells was observed by TEM (B). The expressions of LC-3II/LC-3I and p26 in cells in the LPS + AMSC-Exo-342 group at 4 h, 8 h, 12 h, 24 h, and 36 h were measured by western blot (C, D). The protein expressions of autophagy-related proteins LC3-II/LC3-I, p62, and Beclin-1 were detected by western blot (F-H). ELISA analyzed the contents of inflammatory cytokines TNF-α (I), IL-1β (J), IL-6 (K), and MCP (L) in the supernatant of HK-2 cells. * P < 0.05, ** P < 0.01, *** P < 0.001, compared to the Control, LPS, LPS + AMSC-Exo-67, or LPS + AMSC-Exo-342 group; AMSCs, adipose-derived mesenchymal stem cells; Exo, exosome; LPS, lipopolysaccharide; Rapa, Rapamycin; 3-MA, 3-Methyladenine; TEM, transmission electron microscope. Data were analyzed using one-way analysis of variance and Tukey test was used for post hoc analysis. Data were generated from three independent experiments

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