Fig. 1From: Exosomal microRNA-342-5p secreted from adipose-derived mesenchymal stem cells mitigates acute kidney injury in sepsis mice by inhibiting TLR9Expressions of miR-342-5p and TLR9 in AKI. Notes: qRT-PCR measured the mRNA expression of miR-342-5p in the serum of healthy volunteers and sepsis-associated AKI patients (A). The levels of BUN (B) and SCr (C) in serum of healthy volunteers and AKI patients were detected by ELISA. After construction of AKI cell models, the expressions of miR-342-5p (D) and TLR9 (E, F) were examined by qRT-PCR and western blot. Immunofluorescence assessed the fluorescence intensity of LC3 in HK-2 cells (G), and TEM observed the number of autophagosomes in HK-2 cells (H, I). Then, western blot was used to inspect the expressions of autophagy-related proteins LC3-II/LC3-I, p62 and Beclin-1 (J-M), and ELISA to evaluate inflammation-related factors TNF-α, IL-1β, IL-6, and MCP-1 in the supernatant of HK-2 cells (N-Q); N = 3, ** P < 0.01, compared to the Normal/control group; * P < 0.05, ** P < 0.01, *** P < 0.001, compared to the Control group; AKI, acute kidney injury. Data were analyzed by the two-tailed T test. Data of in vitro experiments were generated from three independent experimentsBack to article page