Fig. 3

Macrophages are related to FC rupture. A Immunohistochemical staining for COL1A1 of the ruptured FC (n = 3). B Immunohistochemical staining for CD163 of the ruptured FC (n = 3). C Immunohistochemical staining for α-SMA of the ruptured FC (n = 3). D Immunohistochemical staining for COL1A1 of the intact FC (n = 3). E Immunohistochemical staining for CD163 of the intact FC (n = 3). F Immunohistochemical staining for α-SMA of the intact FC (n = 3). G CD163 expression in the ruptured FC area was found to be considerably higher than that in the intact FC area (P = 0.0015). H α-SMA expression did not directly correlate with the presence or lack of FC rupture (P = 0.6778). HCC tumor tissues are indicated by T and the paracancerous liver tissues are indicated by N. The red line adjacent to the cancerous tissue is FC; the red arrow indicates the FC ruptured area whose direction is the direction of tumor cells breaking through the ruptured area of FC to invade the paracancerous liver tissue; RFC indicates the ruptured fibrous capsule; IFC indicates the intact fibrous capsule