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Fig. 4 | Biological Procedures Online

Fig. 4

From: Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation

Fig. 4

LNA oligonucleotide blocking of mtrRNA fragments (mitochondrially encoded 16S rRNA (two fragments), 45S pre-ribosomal RNA and mitochondrially encoded 12S rRNA) in QuantSeq 3′ end sequencing. A Counts per million (CPM) of the targeted transcripts for noLNA and LNA100x samples after QuantSeq 3′ end sequencing. Lines are drawn between samples originating from the same lysate. B Average number of detected genes for subsampled noLNA and LNA100x samples. A gene is ‘detected’ if it has at least 10 counts. Each point is a biological replicate and is colored by treatment. C Scatter plot between noLNA and LNA100x samples for each biological replicate are highly correlated (Spearman = 0.853–0.905). Each dot corresponds to a gene. The green dots are MT-RNR1 and MT-RNR2, while the red dots indicate the genes likely affected by off-target binding: H4C3 and MT-ATP8

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