Fig. 2

Blocking of RNY4 transcripts in platelet-rich and platelet-free plasma using different blocking LNA concentrations. A Read percentages for the YRNA and miRNA biotype without the LNA (noLNA) blocking and by adding the LNA in two different concentrations (LNA1x, LNA10x) to both platelet-rich (PRP) and platelet-free (PFP) plasma. B Correlation plots between technical replicates for the blocking strategies and samples. Each dot represents a miRNA. The dots are colored orange when the miRNA had less than 5 counts in either of the replicates. C Correlation plots of the counts between treated (LNA1x) and untreated (no LNA) samples. Only technical replicate 1 is shown here due to the high technical reproducibility. D & E The mean counts per million (CPM) over the technical replicates for all detected (CPM > 0.5) miRNAs and overlap between the detected miRNAs in the LNA blocking (LNA1x) and no blocking condition (noLNA) for both PFP and PRP