Fig. 4
From: Ultrasound and microbubble-mediated delivery of miR-424-5p has a therapeutic effect in preeclampsia
miR-424-5p enhances invasive and migratory behaviors of trophoblast cells through inhibiting AOC1. Note: After HTR-8/Svneo and TEV-1 cells were transfected with miR-424-5p mimic or miR-424-5p mimic + AOC1 overexpression vectors, AOC1 expression was determined by qRT-PCR (A) and western blotting (B); the proliferation rate of transfected cells was assessed by EdU staining (C) and the apoptosis rate by flow cytometry (D); Scratch assay detected the migration capacity of trophoblast cells (E); invasiveness of trophoblast cells were measured by Transwell invasion assay (F); ELISA examined the levels of PLGF, β-hCG and TNF-α in the supernatant (G). * P < 0.05, **P < 0.01, ***P < 0.001. EdU, 5-Ethynyl-2’-deoxyuridine; PLGF, placental growth factor; β-hCG, human chorionic gonadotropin; TNF-α, tumor necrosis factor-α