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Fig. 3 | Biological Procedures Online

Fig. 3

From: Ultrasound and microbubble-mediated delivery of miR-424-5p has a therapeutic effect in preeclampsia

Fig. 3

AOC1 is a target gene of miR-424-5p. Note: After HTR-8/Svneo and TEV-1 cells were transfected with miR-424-5p mimic or mimic NC, qRT-PCR (A) and western blotting (B) were used to detect AOC1 expression; the binding of miR-424-5p and AOC1 to the anti-Ago2 antibody or the negative control anti-IgG antibody in HTR-8/Svneo and TEV-1 trophoblast cells was shown by RIP assay (C); the binding sites of miR-424-5p in the 3’UTR of AOC1 were predicted by TargetScan (D); dual luciferase reporter assay identified the binding of miR-424-5p and AOC1 (E). * P < 0.05, **P < 0.01, ***P < 0.001. RIP, RNA immunoprecipitation; UTR, untranslated region; Ago2, argonaute-2; IgG, immunoglobulin G

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