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Fig. 2 | Biological Procedures Online

Fig. 2

From: Ultrasound and microbubble-mediated delivery of miR-424-5p has a therapeutic effect in preeclampsia

Fig. 2

Overexpression of miR-424-5p promotes the proliferation, invasion and migration of trophoblast cells. Note: After transfection of miR-424-5p mimic or mimic NC into HTR-8/Svneo and TEV-1 cells, qRT-PCR determined the expression of miR-424-5p in HTR-8/Svneo and TEV-1 cells (A); trophoblast cell proliferation was detected through EdU staining (B); flow cytometry measured cell apoptosis rate (C); the migration and invasion capacities of trophoblast cells were assessed by Scratch assay (D) and Transwell invasion assay (E), respectively; the concentrations of PLGF, β-hCG and TNF-α in the supernatant were examined by ELISA (F). * P < 0.05, **P < 0.01, ***P < 0.001. EdU, 5-Ethynyl-2’-deoxyuridine; PLGF, placental growth factor; β-hCG, human chorionic gonadotropin; TNF-α, tumor necrosis factor-α

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