Fig. 5

Validation of differential expression of 5 signature genes in cells and tissues by q-PCR. A-E Differential mRNA expression of 5 signature genes in 7702, LM3, 97H, HepG2 and 7721 cells. The results showed that the expression of CBX2, CDKN2B, HMGA1 and UBE2S in HCC cells was significantly higher than that in normal liver cells. In contrast, the expression of ETS2 was higher in normal liver cell. F-J Differential mRNA expression of 5 signature genes in HCC tissue and paraneoplastic tissue (30 pairs), The expression of CBX2, CDKN2B, HMGA1 and UBE2S were significantly higher in HCC and paraneoplastic tissues. In contrast, ETS2 was lower expressed in HCC tissues