Fig. 4From: Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samplesCytometric gating strategy for the evaluation of frequencies and activation status of sputum leukocytes. Singlets (A), viable cells (B), total leukocytes (C) and total granulocytes/lymphocytes (D) are sequentially selected and then total lymphocytes and granulocytes are plotted in a SSC-A/CD11b-FITC dot-plot (E). Next, total granulocytes (red gate in E) are plotted in a CD16-PCy5/CD11b-FITC dot-plot and gates for selecting CD16High mature neutrophils and CD16Low neutrophils + eosinophils are defined (F). Finally, CD16Low neutrophils + eosinophils (red gate in F) are plotted in a SSC-A/FSC-A dot-plot and SSCHigh eosinophils are selected (G). Cell frequencies and the MFI of membrane markers (percentages and histograms, respectively, in panels E to G) are evaluated in total lymphocytes (pink) mature neutrophils (green) and eosinophils (blue) gatesBack to article page