Table 2 Summary of next-generation sequencing platforms available in the market with their respective manufacturers

1

Roche

454 FLX Titanium

Emulsion PCR on micro beads

Pyro-sequencing

10 h

450 MB

1Million per plate

Modal 450 bp, max.600 bp read lengths

454 FLX + 

23 h

700 MB

1Million per plate

Modal 700 bp, max.1000 bp read lengths

454 GS Junior

10 h

35 MB

0.1 Million per plate

∼450 bp read lengths

2

Illumina

Illumina GAIIx

Flow cell surface used for Bridge-PCR

Reversible terminator sequencing-bysynthesis

6 days

900-1000 GB

250 Million per lane

HiSeq2000/2500 (high output mode): max.2 × 125 bp read lengths

Illumina HiSeq1000

40 h

250–300 GB

125–150 Million per lane

HiSeq 2500(rapid run mode): max.2 × 250 bp read lengths

Illumina HiSeq1500

55 h

13.2–15 GB

22–25 Million per flow cell

MiSeq: 2 × 300 bp read lengths

Illumina HiSeq2000

29 h

100–120 GB

400 Million per flow cell

NextSeq: 500(high output mode) with max. 2 × 150 bp read lengths

Illumina HiSeq 2500

26 h

32–39 GB

130 Million per flow cell

NextSeq 500 (midoutput mode) with max. 2 × 150 bp read lengths

Illumina MiSeq

8 h

15 GB

25 Million per flow cell

2 × 300 bp read lengths

Illumina NextSeq550

12–30 h

120 GB

400 Million

2 × 150 bp read lengths

Illumina HiSeqX

 < 3 days

1.6–1.8 Tb

2.6–3 Billion

2 × 150 bp read lengths

Nova Seq 6000

13-44 h

80-6000 Gb

1.6–40 Billion

2 × 250 bp read lengths

Life Technologies

SOLiD4

Emulsion PCR was done on micro-beads; PCR on Flow Chip surface for the 5500 W models

Sequencing-by-ligation

8 days

260 Gb

160 Million per lane

SOLiD5500

8 days

320 Gb

256 Million per lane

SOLiD5500xl

SOLiD 5500 xl: max.60 bp + 60 bp read lengths

SOLiD5500W

SOLiD5500xl W

SOLiD 5500xlW:max.2 × 5 0 bp read lengths

Life Technologies

Ion PGM

Emulsion PCR on microbeads

Semiconductor dependent sequencing-by-synthesis

7.3 h

1.2–2 Gb

4–5.5 Million per chip

IonPGM(318): max.400 bp read length

Ion Proton(318): 200 bp read length

Life Technologies

Ion Proton

Emulsion PCR on microbeads

Semiconductor dependent sequencing-by-synthesis

2-4 h

Upto10Gb

60–80 Million per chip

IonPGM(318): max.400 bp read length

Ion Proton(318): 200 bp read length

Pacific Biosciences

PacBioRS

Information not provided

Single-molecule, real-time DNA sequencing-by-synthesis

2–3 h per cell

400 Mb per cell

0.05 per SMRT cell

Chemistry used C2/P4 ~ 8000 bp mean read length

Oxford Nanopore

Flongle

Information not provided

Nanopore sequencing

1 min-16 h

2.8 Gb

126 channels per flow cell

 > 4 Mb read length

MinIONMk

1 min-72 h

50 Gb

512 channels per flow cell

 > 4 Mb read length

PromethION

1 min-72 h

14 Tb

2675 channels per flow cell

 > 4 Mb read length

GridIONMk

1 min-72 h

250 Gb

512 channels per flow cell

 > 4 Mb read length