Fig. 4

Mitochondria disgorged Endo G in narrow space. (A) Following treatment of miR-965, refractive index images of MDA-MB-453 were immunostained to detect Endo G (orange; indicated by arrow) at 60 or 66 min. Adjacent cells were digitally colored in red or green and named R and G. (B) ENDOG expression was measured using RT-PCR at 60 or 66 min. (C) Levels of Endo G miRNA in MDA-MB-453 treated with ENDOG CRISPR-plasmid ( +) or control CRISPR-plasmid (-). (D) Mitochondria isolated from Endo G knockout cells were passed through microfluidics into Endo G deficient MDA-MB-453. Following knock down of Endo G, apoptosis (E) and cleaved caspase-3 (F) were determined using ELISA kit and (G-J) the level of cytochrome C, Endo G, or Mcl-1 were determined using western blotting. Results are the means ± SE of 6 experiments in each group. *Significantly different from treatment of unsealed mitochondria following miRNA negative control (NC), P < 0.05. ^#Significantly different from treatment of unsealed mitochondria from Endo G + / + cells, P < 0.05