Fig. 2

Apoptosis of MDA-MB-453 in response to crustacean miRNA. Following treatment with (A) synthetic miR-965 or (B) its antagomir, cell images were 3D rendered with top views by tomographic microscopy at 0, 4, or 5 h. Adjacent cells were digitally stained in green or red. Cells were fixed up to 5 h and apoptosis (C) and caspase-3 (D) were determined using ELISA kit. (E–H) Proteins were extracted from MDA-MB-453 cells and expression of cytochrome C, Endo G, or Mcl-1 was determined using western blotting. Results are the means ± SE of 6 experiments in each group. *Significantly different from treatment of miRNA negative control (NC), P < 0.05. ^#Significantly different from treatment of miR-965, P < 0.05