Fig. 5

Overexpression of circ_0003570 represses tumor growth and lung metastasis in vivo. A The transfection efficiency of HepG2 cells stably transfected with LV-circ_0003570 was detected by qRT‐PCR, and the result showed that the expression of circ_0003570 was evidently overexpressed. **P < 0.01. B Representative images of nude mice modles and formed tumors that were subcutaneously injected with LV- circ_0003570 and NC cells. C, D Effect of circ_0003570 overexpression on HCC growth in vivo according to the tumor growth curve and tumor weight. *P < 0.05; **P < 0.01. E Representative images of HE and IHC staining patterns for STARD13 in tumor xenografts of nude mice (Left:10 × ; Right: 40 ×). F, G Representative images of pulmonary metastatic models and HE staining of metastatic nodules in the lungs (20 ×). Black arrows indicate metastatic nodules. **P < 0.01.Student’s t-test was performed to compare the difference between two groups