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Fig. 3 | Biological Procedures Online

Fig. 3

From: Establishment and Fractionation of Metastatic Axillary Lymph Node Cell Suspension for Determination of Protein Expression Levels of Nuclear cFOS and Cytosolic TGFβ1 from Breast Cancer Patients

Fig. 3

Test of internal control: to verify that the detected cFOS and TGFβ1 markers were derived from HeLa cell nucleus and cytoplasm, respectively. Histograms represent the two time points (t = 2 & t = 4 hrs) selected from time curves presented in Fig. 2 and the two markers measured in nuclear extracts (designated as N2& N4) and cytosolic extracts (designated as C2&C4): A cFOS oncoprotein levels in nuclear versus cytosolic HeLa cell extracts and B TGF-β1 levels in nuclear versus cytosolic HeLa cell extracts

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