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Fig. 2 | Biological Procedures Online

Fig. 2

From: Establishment and Fractionation of Metastatic Axillary Lymph Node Cell Suspension for Determination of Protein Expression Levels of Nuclear cFOS and Cytosolic TGFβ1 from Breast Cancer Patients

Fig. 2

HeLa cell fractionation model illustrated by time curves after serum treatment for: A nuclear cFOS protein, presented by black circles (), and B cytosolic TGF β1 protein, presented by black triangles (). The extracts were obtained by fractionation of growing HeLa cells as described in Methods. All the time points were simultaneously processed and assayed in a single cFOS or TGF-β1 ELISA microplate. Each point was run in duplicate and represents the mean value of two wells. The intra-assay (n = 6) and inter-assay (n = 4) reproducibility was 7 and 15%, respectively

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