Programmable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease

Table 3 Comparison of major platforms incorporating CRISPR/Cas 12

System name	Effector	Recognition type	Sensitivity	Specificity	Signal amplification	Quantitative	Multiplex	Time	Readout	Infrastructure requirement	Reference
CaT-SMelor	LbCas12a	Small molecule	nM	Single molecule	N	Y	N	40 min	Fluorescence	Substantial	[59]
OCTOPUS	LbCas12a	dsDNA	One copy	1 nt	RPA	Y	N	50 min	Fluorescence	Substantial	[60]
CORDS	LbCas12a	ASFV	aM	1 nt	RAA	N	N	~ 1 h	LFA	Few	[61]
Cas12aVDet	LbCas12a	Mycoplasma	aM	1 nt	RPA	N	N	~ 30 min	Naked eye	Few	[25]
CDetection	AaCas12b	HPV	aM	1 nt	RPA	N	N	~ 2.5 h	Fluorescence	Substantial	[62]
CRISPR-MTB	LbCas12a	TB	98%	1 nt	RPA	N	N	/	Fluorescence	Substantial	[63]
DETECTR-COVID19	LbCas12a	SARS-CoV-2	95%	1 nt	RT-LAMP	N	N	~ 45 min	LFA	Few	[19]

CaT-SMelor: CRISPR-Cas12a- and aTF-mediated small molecule detector. OCTOPUS: One-pot toolbox with precision and ultrasensitivity; higher sensitivity than general qPCR; LFA: Lateral flow assay; ASFV: African swine fever virus; CORDS: Cas12a-based on-site and rapid detection system; Cas12aVDet: Cas12a-based visual detection; CRISPR-MTB: Clinic test; CDetection: Cas12b-mediated DNA detection; DETECTR: CRISPR trans reporter

ISSN: 1480-9222