Fig. 2From: Evaluation of the Function of Probiotics, Emphasizing the Role of their Binding to the Intestinal Epithelium in the Stability and their Effects on the Immune SystemSortase mechanism of action in binding SDPs to cell wall. A SDPs are distinguishable with The presence of an N-terminal signal peptide and a C-terminal LPXTG sorting signal, followed by hydrophobic and positively modified residues that facilitate membrane anchoring, distinguishes sortase substrates. B In a sequence of five processes, SDPs are linked to the cell wall;1. sec machinery recognizes the signal peptide on the SDPs and exports it to the cell’s exterior. 2: When sortase and the SDPs are in close proximity, sortase cleaves the SDP between the glycine and threonine residues with transpeptidase action.3 The sortase/SDP complex is dissociated by lipid II’s nucleophilic attachment and 4: Through contact with the pentapeptide cross bridge, it produces a lipid II intermediate.5: As part of regular cell wall construction, the sortase substrate is integrated into the cell wallBack to article page