Fig. 4

The area corresponding to the 3D image is indicated by the red square line (A). The main L.V.(white colored) runs along the sides of the tail and the lateral veins (blue colored) and its distribution is also shown (B). The ventral artery runs along the tail ((B): red colored). Total number of 533 serial sections of fluorescent stained (either CD31 or LYVE1) images were incorporated as digital data and processed by Amira software to construct 3D images of edema and its adjacent regions (C, D). In more proximal side from the operated region (adjacent to the base of the tail), generation of fine blood vessel was observed by green signal ((C): in dotted white circle region). As for the operated region close to the edema, the presence of two main enlarged L.V. by surgical ligation was clearly observed ((D); yellow circle region). Fine (neo) hyperplastic L.V. were observed by the purple signals ((D): white circle region), fine (neo) blood vessel formation was observed by the green signals ((D): dotted white circle region)