Fig. 3

Fluoresce images were acquired with light sheet fluorescence microscopy (LSFM), (ZEISS Z.1, Germany). The LSFM system set up is shown (A). PFA fixed specimen was placed at sample fold (green circle) with Excitation light (yellow arrow) toward the detection device by Emission light (blue arrow). The distribution of FITC dextran signal was suggested the presence of main L.V. (shown by two white arrow) and presence of presumptive fine (neo) L.V. (shown by white dotted arrow in (B)). It was suggested that main and fine (neo) L.V. tended to extend to the ligation site (yellow mark, (B))