Table 2 Summary and classification of the articles by using scaffold to induce odontoblast

1

Soares et al

2017

- Healthy human third molars

- hDPSCs were seeded on NF-PLLA scaffolds that mimic the nanofibrous architecture of extracellular matrix, and cultured with simvastatin and/or pro-inflammatory stimulator LPS

- Treating LPS + DPC/NF-PLLA constructs with simvastatin reverted negative effects of LPS on expression of odontoblastic markers, associated to the reduction in NFkBp65 phosphorylation and upregulation of PPARγ expression

- The DPC/NF-PLLA constructs treated with LPS/simvastatin increased vessel-like structures, which corelated to increased VEGF expression in the DPSCs

- Combination of low dosage simvastatin and NF-PLLA scaffolds promotes dentin regeneration in inflamed dental pulp tissue

2

Yuanwei Chen et al

2015

- hUCMSCs

- TGC-CM was added to induce hUCMSCs into odontoblast-like cells

- Preparation of hTDM by staining with HE and Masson’s trichrome

- After the preparation of hTDM, hUCMSCs were differentiated under the odontogenic microenvironment provided by hTDM

- Induction of hUCMSCs with TGC-CM upregulate the expression of DSP and DMP-1

- hTDM was positive for DSPP and DMP-1 especially around dentin tubules, indicating that the dentin expressed DSPP and DMP-1

- hUCMSCs have an odontogenic differentiation potential to differentiate into odontoblast-like cells in an odontogenic microenvironment provided by TGC-CM and hTDM in vitro

3

Chunyang Huang

2020

- hDPSCs

- hUCMSCs

- hDPCs and hUCMSCs were cultured in different concentrations of hydrogel to explore the more suitable concentrations for subsequent experiments

- hUCMSCs and hDPCs are viable and able to proliferate in 0.25% hydrogel scaffold

- Compared with hUCMSCs-monoculture and hDPCs-monoculture, the co-culture groups exhibited more proliferative potential, alkaline phosphatase activity and mineralization nodule formation (P < 0.05)

- The co-culture of HUCMSCs and hDPSCs in hydrogel scaffold could regulate cell proliferation and differentiation within a certain range

4

Mohammad Chair Effendi et al

2015

- SHEDs

- SHED was cultured with MTA dose 2 mg and NMT dose 2 mg

- NMT was non-toxic towards SHED and increased the proliferation of SHED, and did not impede SHED viability especially on the second day compared to MTA

- NMT could increase activity of ALP and DSPP compared to MTA on the SHED

- NMT could increase proliferation of SHED, increased ALP and DSPP activity in SHED and did not impede SHED viability

5

Tang, J. et al

2015

- MDPC-23 from foetal mouse first molar papillae

- Biocompatibility of type I collagen was evaluated in terms of initial cell number, ALP activity ALP activity, odontogenic gene expression and calcific deposition

- Cells cultured in type I collagen-modified substrate was induced to differentiate toward odontoblast lineage as demonstrated by upregulation of ALP activity on day 7, enhancement of ALP, BSP mRNA expression on day 7 and 10, and accelerated mineralization on day 9

- TS collagen accelerated early and late stage cellular differentiation as evidenced by enhancement of ALP activity and promotion of BSP, ALP, and OCN mRNA expression

- Mineralization was dramatically accelerated in cells cultured on TS collagen

6.

Shunro Miyashita et al

2017

- hDPSC (first premolar and third molar, 14–28 years old)

- hBMMSC

- hAMC

- Odontoblastic differentiation in response to mechanical compression of three-dimensional scaffolds with

dentinal tubule-like pores

- Cell density of 4.0 × 105 cells/cm2;

compression magnitude of 19.6 kPa; and loading time of 9 h were considered optimal conditions for differentiation

- hDPSCs cultured on 3D scaffolds significant increase expression of DSPP and enamelysin

- Expression of DSPP and enamelysin in hBMMSCs and hAMCs with mechanical compression were similar to those in hDPSCs

- It may be possible to differentiate even the other tissue-derived

hMSCs into odontoblasts by mechanical compression on membranes with pores like dentinal tubes