Fig. 6From: Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing SystemDetachment and purification of captured cells. A A schematic overview of a 2-step cell purification protocol. In the first step, captured cells were invertedly centrifuged to remove the weakly-bound cell. In the second step, remaining cells were detached by treatment of trypsin. B Expression of ACE2 on transiently transfected ACE2-293 T cells before (left panel) and after (right panel) purification and gate M1 indicates the proportion of cells with high expression of ACE2-GFP. C Cell viability of ACE2-293 T cells before and after the purification. Cell viability was determined using Annexin-V/PI stainingBack to article page