Fig. 5

The D614G mutation increases ACE2-expressing cell capturing ability of S protein. A S1 or D614G S1 variant was immobilized on 96 well microplates, and HEK293 cells stably expressing ACE2-GFP were incubated with ACE2-293 T cells. Captured cells were detached by Trypsin and counted by CCK8 test. Quantitation data are presented as mean ± SD; *** p < 0.05 by unpaired Student t-test. B FLAG-tagged ACE2 stable expressed cells lysate were incubate with Spike S1 or S1(D614G) proteins. After FLAG-IP, the immunocomplexes were blotted with anti-FLAG or anti-spike antibodies as indicated. Spike S1 monomer (apparent molecular weight ~ 116Kd) and trimer (apparent molecular weight ~ 300Kd) were found after the western blotting. Fluorescence intensity quantification of S1 or S1(D614G) indicated below each band were normalized by each input.