Fig. 4

RT-PCR analysis of two ALP-positive HDDPCs collected through RNA-snMIFxC. (A) Two alkaline phosphatase (ALP)-positive HDDPCs sorted. Bar = 100 μm. (B) RT-PCR analysis of HDDPC-ALP( +) using primer sets for ALP, octamer-binding transcription factor 3/4 (OCT3/4), sex determining region Y-box 2 (SOX2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). M, 100 bp ladder; -RT, PCR performed in the absence of reverse transcription products. HDDPC PC, RT-PCR products using cDNAs derived from unselected HDDPCs