Fig. 3From: Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptorsAbundance indicated by qPCR and ddPCR. Ct values measured by qPCR were plotted against the corresponding copies per µl measured for the identical samples by ddPCR. The resulting negative-exponential relationship is highlighted by a trend line. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates eachBack to article page