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Fig. 1 | Biological Procedures Online

Fig. 1

From: Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors

Fig. 1

Effect direction indicated by qPCR and ddPCR. Human endothelial cells of the cell line TIME (ATCC number: CRL-4025) were stimulated for 24 h with TNF-α, IL-1β, and INF-γ in concentrations of 100 ng/ml each. The measured values are presented as mean values ± standard deviation. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates each. * symbolizes significant differences. ACE1 = angiotensin converting enzyme 1, ADRA1B = alpha-adrenergic receptor type 1B, ADRA1D = alpha-adrenergic receptor type 1D, ADRB2 = beta-adrenergic receptor type 2, ATIP1 = angiotensin II receptor interacting protein 1, ATRAP = angiotensin II type 1 receptor associated protein, EDNRA = endothelin receptor type A, EDNRB = endothelin receptor type B

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