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Fig. 8 | Biological Procedures Online

Fig. 8

From: A Practical Guide to Rodent Islet Isolation and Assessment Revisited

Fig. 8

Calcium response to glucose stimulation. a Calcium traces recorded at 10-s intervals during a series of increasing glucose steps (0, 4, 8, 12, 16, 20 mM) in an 90-min recording (N=20 islets). For each glucose step both 1st phase (peak) and 2nd phase (plateau, measured in the last 5-min) were measured. b-c Mean values for each phase at each glucose step are used to assemble glucose dose-response curves for 1st phase (b) and 2nd phase (c) calcium responses as an indicator of islet function

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