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Fig. 7 | Biological Procedures Online

Fig. 7

From: A Practical Guide to Rodent Islet Isolation and Assessment Revisited

Fig. 7

An example of thapsigargin-induced and cytokine-induced cell death as measured by annexin V and propidium iodide fluorescence. a A brightfield image of healthy mouse islets (top), islets treated overnight with 100 nM thapsigargin to induce ER stress (middle), and islets treated overnight with 5 ng/mL IL-1beta and 10 ng/mL TNF-alpha (cytokines) to induce cell death (bottom). b Annexin V is depicted with green fluorescence and propidium iodide is depicted with red fluorescence (c)

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