Fig. 5
From: A Practical Guide to Rodent Islet Isolation and Assessment Revisited
Islets after optimal digestion and recovery time. a An example of islets immediately post-isolation and purification. b-c The same preparation of islets after a two-hour recovery period but before cleaning (b) and after overnight recovery and before a final cleaning (c). Note that 88 islets were counted in (b) and 80 islets in (c) even though the tissue density appears quite different between images. Pancreatic debris accounts for the additional tissue observed in (b) prior to final cleaning