Table 5 Primer sequences used for sequencing and PCR reactions
Allele | Application | Primer | Sequence (5′-3′) | Marker |
|---|---|---|---|---|
ACE*16 | Sequencing | *16-seq-F | CTGGAGAGCCACTCCCATCCTTTCT | |
*16-seq-R | GACGTGGCCATCACATTCGTCAGAT | |||
PCR reactions | *16-F | AAGGAGAGGAGAGAGACTCAAGCAC | 5′-labeled biotin | |
*16-R (M) | TCGAGACCATCCCGGCTAAAAC | 5′-labeled digoxin | ||
*16-R (WT) | AACCACATAAAAGTGACTGTATCGG | 5′-labeled digoxin |